Human papillomavirus type 58 infection and integration in exfoliated cervical cells / 西安交通大学学报(医学版)

ABSTRACT

【Objective】 To detect the types of human papillomavirus （HPV） infection in exfoliated cervical cells to screen patients with early cervical lesions through HPV infection； analyze the viral load and integration sites of HPV58 positive specimens to explore the relationship of HPV58 viral load and integration status with cervical lesions in the hope of providing a scientific basis for the prevention and treatment of cervical lesions. 【Methods】 The exfoliated cervical cells and their clinicopathological data were collected， and genome DNA and total RNA were extracted from the collected specimen. HPV infections were detected using MY09/11 of HPVL1 region general primers and HPV58 was detected using HPV58 E6 specific primers. The copy numbers of HPV58 E6， E2 and human housekeeper gene β-actin were detected by quantitative fluorescence PCR， and the integration status of HPV58 was determined by the ratio of E2 to E6. At the same time， the position of HPV58 integrated into the host chromosome was analyzed through oncogene transcription amplification. 【Results】 Totally 295 HPV positive cases were detected in 1 269 exfoliated cervical cell samples， with the total infection rate of 23.2%. Among them 34 HPV58 positive were detected， which consisted of 4 samples of pure episome， 8 samples of integration， and 22 cases of mixture （both integration and episome）. Statistical analysis showed no significant correlation of the degree of cervical lesions with the viral load and integration status of HPV58. Eight HPV58 samples were integrated into chromosomes 1， 2， 5， 8， and 17， respectively. 【Conclusion】 HPV positive rate was 23.2% in the exfoliated cervical cells， the HPV infection rate increased with the aggravation of cervical lesions. Patients with early cervical lesions can be screened through high-risk HPV infection.