Content measurement of doxorubicin hydrochloride and lonidamine by HPLC / 药学实践与服务
Journal of Pharmaceutical Practice and Service
; (12): 127-130, 2024.
Article
em Zh
| WPRIM
| ID: wpr-1012793
Biblioteca responsável:
WPRO
ABSTRACT
Objective To establish a method for the simultaneous determination of DOX·HCl and LND. Methods HPLC was performed on Agilent 5 HC-C18(2) (4.6 mm × 250 mm, 5 µm) column. The mobile phase was methanol-0.1% TFA aqueous solution, and the gradient elution procedure were: 0 to 3 min, 65% methanol; 3 to 7 min, 65%→90% methanol; 7 to 13 min, 90% methanol; 13 to 15 min, 90%→65% methanol; 15 to 20 min, 65% methanol. The collection time was 20 min, the balance time was 3 min, the UV detection wavelengths were 205 nm and 253 nm. The flow rate was 1.0 ml/min and the column temperature was 35℃. The amount of inlet was 10 µl. Results The method was highly specific, and both DOX·HCl and LND exhibited good linearity in the concentration range of 1-40 µg/ml and 6-240 µg/ml, respectively. The two compounds’ precision, stability, and recovery satisfied the requirements of the method. Conclusion This study established a HPLC method that was suitable for the simultaneous detection of DOX·HCl and LND. This method’s high level of specificity, accuracy, and reliability .
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1
Base de dados:
WPRIM
Idioma:
Zh
Revista:
Journal of Pharmaceutical Practice and Service
Ano de publicação:
2024
Tipo de documento:
Article