Mechanism of citizene improving insulin resistance of HepG2 cells based on KDSR gene / 中国药理学通报

ABSTRACT

Aim To explore whether Huaqizeren, an experienced prescription for the treatment of IR, could improve IR by interfering with KDSR expressionfrom the perspective of KDSR gene. Methods The KDSR gene was overexpressed in normal HepG2 cells, and the IR cell model was established at the same time. The expression level of KDSR was detected by QRT PCR and Western blot; the content of ceramide was detected by HPLC-MS; the expression level of KDSR was detected by Western blot. The phosphorylation level of PKC£/Akt/Fox01 was detected by Western blot, and the glucose content was detected by GOD-POD assay before and after administration. Results After administration, the expression level of KDSR and ceramide in IR cells and KDSR overexpression cells significantly decreased, and the abnormal expression of PKC£/Akt/ FoxOl signaling pathway was improved. Meanwhile, the glucose content in IR cells and KDSR overexpres-sion cells significantly decreased after administration, and the differences were statistically significant (P < 0. 01). Conclusions One of the mechanisms of Huaqizeren in improving IR may be down-regulating the expression of KDSR, decreasing the content of ceramide , and then regulating the expression of key proteins in PKC£/Akt/Fox01 signaling pathway, thus reducing the glucose content in cells and improving IR.