Fabrication of a Scaffold for Bone Regeneration and Differentiation of Mesenchymal Stem Cells / 대한정형외과연구학회지

ABSTRACT

PURPOSE: This in-vitro study aimed to confirm the clinical potential of a newly suggested bone substitute fabricated by a bio-plotting system. MATERIALS AND METHODS: A 3-dimensional, PCL (polycaprolactone)-based bone substitute was fabricated by a bio-plotting system, which enables us to control the shape and porosity. Mesenchymal stem cells(MSCs) from a New Zealand white rabbits were seeded to the bone substitute. Through 2-week experiment, morphological and various biological responses were assessed depending on the use of osteogenic media and/or mechanical stimuli. RESULTS: The viability of cells was confirmed. Other findings in relation to proliferation and differentiation were also confirmed to be biocompatible. Specifically, the activity of ALP was increased in the groups using osteogenic media along the time. And the expressions collagen type I was more affected by intermittent hydrostatic pressure rather than by osteogenic media. But those of Cbfa-1 was affected by osteogenic media and mechanical stimuli, as well. CONCLUSION: The potential of a newly fabricated and suggested material was confirmed. However, further long term and animal study are recommanded for the clinical application.