Changes of Immune Cells in the Evolution of Myelodysplastic Syndromes / 医学研究杂志

ABSTRACT

Objective To observe the expression differences of peripheral blood immune cells in healthy people,myelodysplastic syndrome(MDS)patients and acute myeloid leukemia(AML)patients,and to further analyze the correlation between the differences and International Prognostic Scoring System(IPSS)score,original cell number and other factors in MDS patients.Methods A total of 55 MDS patients admitted to Dongzhimen Hospital,Beijing University of Chinese Medicine from January 2012 to April 2022 were retrospec-tively analyzed,and 25 healthy people and 67 AML patients were included as control groups.The expression of T lymphocyte subsets(CD3+,CD4+,CD8+,CD4+/CD8+,CD3+CD4+CD25+FoxP3+),B cells(CD19+)and NK cells(CD3-CD16+CD56+)were compared according to the absolute count of immune cells and their percentage in lymphocytes.The correlation between the difference in-dex and IPSS score and the number of original cells was analyzed.Results There were differences in the expression levels of immune in-dicators among the three groups.Pairwise comparison indicated that compared with the control groups,the immune cells count of MDS group were lower(P＜0.025),which was considered to be related to the total lymphocyte count in MDS patients were lower than those in the healthy group.The expression levels of immune cells in the three groups were different in the percentage of Treg cells,B cells and T lymphocytes.Pairwise comparison showed that compared with the healthy group,the proportion of T lymphocytes and Treg cells in MDS group was higher,and the proportion of B cells was lower,and the difference between T lymphocytes and B cells was statistically signifi-cant(P＜0.025).Compared with MDS group,the proportion of Treg cells was higher and the proportion of B cells was lower in AML group,the difference was statistically significant(P＜0.025).The proportion of Treg cells was positively correlated with IPSS score and the number of original cells.Conclusion The expression level of Treg cells in healthy people,MDS and AML showed an overall increasing trend.The higher expression level of Treg cells in MDS patients may be an important factor to evaluate the prognosis and progression of MDS patients.