Tracking Intravenous Adipose-Derived Mesenchymal Stem Cells in a Model of Elastase-Induced Emphysema / 결핵및호흡기질환
Tuberculosis and Respiratory Diseases
; : 116-123, 2014.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-103201
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND:
Mesenchymal stem cells (MSCs) obtained from bone marrow or adipose tissue can successfully repair emphysematous animal lungs, which is a characteristic of chronic obstructive pulmonary disease. Here, we describe the cellular distribution of MSCs that were intravenously injected into mice with elastase-induced emphysema. The distributions were also compared to the distributions in control mice without emphysema.METHODS:
We used fluorescence optical imaging with quantum dots (QDs) to track intravenously injected MSCs. In addition, we used a human Alu sequence-based real-time polymerase chain reaction method to assess the lungs, liver, kidney, and spleen in mice with elastase-induced emphysema and control mice at 1, 4, 24, 72, and 168 hours after MSCs injection.RESULTS:
The injected MSCs were detected with QD fluorescence at 1- and 4-hour postinjection, and the human Alu sequence was detected at 1-, 4- and 24-hour postinjection in control mice (lungs only). Injected MSCs remained more in mice with elastase-induced emphysema at 1, 4, and 24 hours after MSCs injection than the control lungs without emphysema.CONCLUSION:
In conclusion, our results show that injected MSCs were observed at 1 and 4 hours post injection and more MSCs remain in lungs with emphysema.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Baço
/
Medula Óssea
/
Tecido Adiposo
/
Doença Pulmonar Obstrutiva Crônica
/
Pontos Quânticos
/
Enfisema
/
Rastreamento de Células
/
Células-Tronco Mesenquimais
/
Reação em Cadeia da Polimerase em Tempo Real
/
Imagem Óptica
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
Tuberculosis and Respiratory Diseases
Ano de publicação:
2014
Tipo de documento:
Artigo