Molecular Characterization of Taenia multiceps Isolates from Gansu Province, China by Sequencing of Mitochondrial Cytochrome C Oxidase Subunit 1
The Korean Journal of Parasitology
; : 197-201, 2013.
Article
em En
| WPRIM
| ID: wpr-103954
Biblioteca responsável:
WPRO
ABSTRACT
A total of 16 Taenia multiceps isolates collected from naturally infected sheep or goats in Gansu Province, China were characterized by sequences of mitochondrial cytochrome c oxidase subunit 1 (cox1) gene. The complete cox1 gene was amplified for individual T. multiceps isolates by PCR, ligated to pMD18T vector, and sequenced. Sequence analysis indicated that out of 16 T. multiceps isolates 10 unique cox1 gene sequences of 1,623 bp were obtained with sequence variation of 0.12-0.68%. The results showed that the cox1 gene sequences were highly conserved among the examined T. multiceps isolates. However, they were quite different from those of the other Taenia species. Phylogenetic analysis based on complete cox1 gene sequences revealed that T. multiceps isolates were composed of 3 genotypes and distinguished from the other Taenia species.
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Texto completo:
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Base de dados:
WPRIM
Assunto principal:
Filogenia
/
Doenças dos Ovinos
/
Taenia
/
Variação Genética
/
DNA Mitocondrial
/
Cisticercose
/
Cabras
/
Ovinos
/
Análise por Conglomerados
/
Doenças das Cabras
Limite:
Animals
País/Região como assunto:
Asia
Idioma:
En
Revista:
The Korean Journal of Parasitology
Ano de publicação:
2013
Tipo de documento:
Article