The significance of CD4+CD28-T cells，TNF-α and VCAM-1 in acute cerebral infarction and its correlation with the properties of carotid plaque / 中风与神经疾病杂志

ABSTRACT

@#Objective　To investigate the changes of CD4+CD28-T lymphocytes subsets，tumor necrosis factor-α（TNF-α） and vascular cell adhesion molecule-1（VCAM-1） in peripheral blood of patients with acute cerebral infarction（ACI） and their correlation with the nature of carotid plaques. Further analysis of the correlation among CD4+CD28-T cells，TNF-α and VCAM-1 in patients with cerebral infarction. Methods　From August 2018 to April 2019，CD4+CD28-T lymphocytes，TNF-α and VCAM-1 in peripheral blood of 57 patients with acute atherosclerotic cerebral infarction,43 patients with acute arterioarterial cerebral infarction and 40 healthy controls were collected and were detected by flow cytometry and ELISA in the Department of Neurology,the Affilated of Shengjing Hospital of China Medical University,and the level of CD4+CD28-T lymphocytes，TNF-α and VCAM-1 in different groups was analyzed. 47 patients with internal carotid artery plaques were obtained by carotid color Doppler ultrasound in 57 patients with acute atherosclerotic cerebral infarction，including 19 patients with stable plaques and 28 patients with unstable plaques. The relationship between CD4+CD28-T lymphocytes，TNF-α，VCAM-1 and atherosclerotic cerebral infarction plaque stability was analyzed. The correlation between them in patients with acute cerebral infarction was determined by scatter plot. Results　The levels of CD4+CD28-T lymphocytes，TNF-α and VCAM-1 in peripheral blood of patients with acute atherosclerotic cerebral infarction were higher than those of patients with arteriole cerebral infarction，and the levels of CD4+CD28-T lymphocytes，TNF-α and VCAM-1 in both groups was higher than those in healthy controls，and there were significant difference among three groups（P<0.05）. The levels of CD4+CD28-T lymphocytes，TNF-α and VCAM-1 in unstable plaque group were higher that in stable plaque group.There was significant difference between the two groups（P<0.05）.At the same time，there was a linear correlation between CD4+CD28-T lymphocytes and TNF-α，VCAM-1 in all patients with acute cerebral infarction. Conclusion　The levels of CD4+CD28-T lymphocytes，TNF-α and VCAM-1 in peripheral blood of patients with acute cerebral infarction during the acute phage were increased，and CD4+CD28-T lymphocytes，TNF-α and VCAM-1 were mainly involved in the formation of atherosclerotic unstable plaques. The levels of CD4+CD28-T lymphocytes were correlated with TNF-α and VCAM-1 in patients with acute cerebral infarction，respectively.