Asian Dust Particles Induce TGF-beta1 via Reactive Oxygen Species in Bronchial Epithelial Cells / 결핵및호흡기질환
Tuberculosis and Respiratory Diseases
; : 84-92, 2012.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-105221
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND:
Asian dust storms can be transported across eastern Asia. In vitro, Asian dust particle-induced inflammation and enhancement of the allergic reaction have been observed. However, the fibrotic effects of Asian dust particles are not clear. Production of transforming growth factor beta1 (TGF-beta1) and fibronectin were investigated in the bronchial epithelial cells after exposure to Asian dust particulate matter (AD-PM10).METHODS:
During Asian dust storm periods, air samples were collected. The bronchial epithelial cells were exposed to AD-PM10 with and without the antioxidant, N-acetyl-L-cysteine (NAC). Then TGF-beta1 and fibronectin were detected by Western blotting. The reactive oxygen species (ROS) was detected by the measurement of dicholorodihydrofluorescin (DCF), using a FACScan, and visualized by a confocal microscopy.RESULTS:
The expression of TGF-beta1, fibronectin and ROS was high after being exposed to AD-PM10, compared to the control. NAC attenuated both TGF-beta1 and fibronectin expression in the AD-PM10-exposed the bronchial epithelial cells.CONCLUSION:
AD-PM10 may have fibrotic potential in the bronchial epithelial cells and the possible mechanism is AD-PM10-induced intracellular ROS.
Texto completo:
Disponível
Contexto em Saúde:
ODS3 - Saúde e Bem-Estar
Problema de saúde:
Meta 3.9: Reduzir o número de mortes por produtos químicos perigosos e contaminação do ar e água do solo
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Acetilcisteína
/
Western Blotting
/
Fator de Crescimento Transformador beta
/
Fibronectinas
/
Espécies Reativas de Oxigênio
/
Povo Asiático
/
Poluentes Atmosféricos
/
Poeira
/
Células Epiteliais
/
Fator de Crescimento Transformador beta1
Limite:
Humanos
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Tuberculosis and Respiratory Diseases
Ano de publicação:
2012
Tipo de documento:
Artigo