Comparative Analysis of Multiplex PCR and Hybrid Capture System in the Detecttion of Human Papillomavirus in the Uterine Cervix / 대한산부인과학회잡지
Korean Journal of Obstetrics and Gynecology
; : 28-33, 2003.
Artigo
em Coreano
| WPRIM (Pacífico Ocidental)
| ID: wpr-113190
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE:
To compare the multiplex-PCR and hybrid capture methods for detection of Human papillomavirus (HPV) in uterine cervical swab samples.METHODS:
This study determined the HPV infection and its risk group (subtype) in uterine cervical swab samples of 91 Korean women by HPV hybrid capture I and multiplex-PCR method.RESULTS:
The prevalence of HPV infection determined by muliplex-PCR was more higher than by hybrid capture method I. Of 65 women with nonspecific uterine cervical lesions, the frequence of HPV-positivity was 14/65 (12 low-risk and 2 high-risk HPV) by hybrid capture method I and was 37/65 (34 low-risk and 3 high- risk HPV) by multiplex-PCR method, respectively. The frequence of high-risk HPV was 2/6 in LSIL, 7/14 in HSIL, 4/6 in uterine cervical cancer by hybrid capture method I, and was 2/6 in LSIL, 8/14 in HSIL, 4/6 in uterine cervical cancer by multiplex-PCR method.CONCLUSION:
These results suggest that multiplex-PCR is more sensitive, simple and cost-effective technique than Hybrid-capture I in the detection of HPV infection in the uterine cervix.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Neoplasias do Colo do Útero
/
Colo do Útero
/
Prevalência
/
Reação em Cadeia da Polimerase Multiplex
Tipo de estudo:
Estudo de prevalência
Limite:
Feminino
/
Humanos
Idioma:
Coreano
Revista:
Korean Journal of Obstetrics and Gynecology
Ano de publicação:
2003
Tipo de documento:
Artigo