Surveyor assay to diagnose persistent Müllerian duct syndrome in Miniature Schnauzers
Journal of Veterinary Science
; : 547-549, 2017.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-11453
Biblioteca responsável:
WPRO
ABSTRACT
Persistent Müllerian duct syndrome (PMDS) is a pseudohermaphroditism in males characterized by the presence of Müllerian duct derivatives. As PMDS dogs often lack clinical symptoms, a molecular diagnosis is essential to identify the syndrome in these animals. In this study, a new molecular method using DNA mismatch-specific Surveyor nuclease was developed. The Surveyor nuclease assay identified the AMHR2 mutation that produced PMDS in a Miniature Schnauzer as accurately as that obtained by using the conventional method based on restriction digestion. As an alternative to the current molecular diagnostic method, the new method may result in increased accuracy when detecting PMDS.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Transtornos do Desenvolvimento Sexual
/
DNA
/
Diagnóstico
/
Digestão
/
Patologia Molecular
/
Métodos
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Journal of Veterinary Science
Ano de publicação:
2017
Tipo de documento:
Artigo