Enhanced Atrial Natriuretic Peptide and Nitric Oxide System Following the Treatment with Caffeine in Rats / 대한신장학회잡지
Korean Journal of Nephrology
; : 123-128, 2002.
Artigo
em Coreano
| WPRIM (Pacífico Ocidental)
| ID: wpr-126469
Biblioteca responsável:
WPRO
ABSTRACT
PURPOSE:
The present study was aimed to examine whether caffeine alters the local regulation of atrial natriuretic peptide(ANP) and nitric oxide(NO) systems in the kidney.METHODS:
Male Sprague-Dawley rats were treated with caffeine, consisting of a single oral bolus(0.2%, 1 mL/kg) followed by supplementation in drinking water(0.2%) for 1 day. Rats treated the same without caffeine served as control. The tissue expression of ANP mRNA was determined by reverse transcription-polymerase chain reaction. Tissue levels of nitrite/nitrate were determined. The expression of aquaporin(AQP)-1 and AQP2 proteins was determined by Western blot analysis.RESULTS:
Following the treatment with caffeine, the expression of ANP mRNA was increased in the kidney. The renal tissue nitrite/nitrate level was also increased by caffeine-treatment. On the other hand, the expression of AQP1 and AQP2 proteins was not significantly altered.CONCLUSION:
The enhanced activities of local ANP and NO systems may in part be causally related with the caffeine-induced natriuresis and diuresis, while AQP channels are not involved.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Cafeína
/
RNA Mensageiro
/
Western Blotting
/
Fator Natriurético Atrial
/
Ratos Sprague-Dawley
/
Diurese
/
Ingestão de Líquidos
/
Aquaporina 2
/
Mãos
/
Rim
Limite:
Animais
/
Humanos
/
Masculino
Idioma:
Coreano
Revista:
Korean Journal of Nephrology
Ano de publicação:
2002
Tipo de documento:
Artigo