Your browser doesn't support javascript.
loading
Efficient Induction of Th1-type Immune Responses to Hepatitis B Virus Antigens by DNA Prime-Adenovirus Boost
Immune Network ; : 1-10, 2005.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wpr-127006
Biblioteca responsável: WPRO
ABSTRACT

BACKGROUND:

Chronic infection with hepatitis B virus (HBV) affects about 350 million people worldwide, which have a high risk of development of cirrhosis and hepatocellular carcinoma. Treatment of chronic HBV infection relies on IFN-alpha or lamivudine. However, interferon-alpha is effective in only about 30% of patients. Also, the occurrence of escape mutations limits the usage of lamivudine. Therefore, the development and evaluation of new compounds or approaches are urgent.

METHODS:

We comparatively evaluated DNA and adenoviral vaccines expressing HBV antigens, either alone or in combined regimens, for their ability to elicit Th1-type immune responses in Balb/c mice which are believed to be suited to resolve HBV infection. The vaccines were tested with or without a genetically engineered IL-12 (mIL-12 N220L) which was shown to enhance sustained Th1-type immune responses in HCV E2 DNA vaccine.

RESULTS:

Considering the Th1-type cytokine secretion and the IgG2a titers, the strongest Th1-type immune response was elicited by the DNA prime-adenovirus boost regimen in the presence of mIL-12 N220L. In addition, the codelivery of mIL-12 N220L modulated differentially the immune responses by different vaccination regimens.

CONCLUSION:

Our results suggest that the DNA prime-adenovirus boost regimen in the presence of mIL-12 N220L may be the best candidate for HBV vaccine therapy of the regimens tested in this study and will be worthwhile being evaluated in chronic HBV patients.
Assuntos

Texto completo: Disponível Contexto em Saúde: ODS3 - Saúde e Bem-Estar / ODS3 - Meta 3.3 Acabar com as doenças tropicais negligenciadas e combater as doenças transmissíveis Problema de saúde: Meta 3.3: Acabar com as doenças tropicais negligenciadas e combater as doenças transmissíveis / Hepatite Base de dados: WPRIM (Pacífico Ocidental) Assunto principal: Nações Unidas / Fibrose / DNA / Imunoglobulina G / Vacinas / Vírus da Hepatite B / Imunoterapia Ativa / Vacinação / Interferon-alfa / Carcinoma Hepatocelular Limite: Animais / Humanos Idioma: Inglês Revista: Immune Network Ano de publicação: 2005 Tipo de documento: Artigo
Texto completo: Disponível Contexto em Saúde: ODS3 - Saúde e Bem-Estar / ODS3 - Meta 3.3 Acabar com as doenças tropicais negligenciadas e combater as doenças transmissíveis Problema de saúde: Meta 3.3: Acabar com as doenças tropicais negligenciadas e combater as doenças transmissíveis / Hepatite Base de dados: WPRIM (Pacífico Ocidental) Assunto principal: Nações Unidas / Fibrose / DNA / Imunoglobulina G / Vacinas / Vírus da Hepatite B / Imunoterapia Ativa / Vacinação / Interferon-alfa / Carcinoma Hepatocelular Limite: Animais / Humanos Idioma: Inglês Revista: Immune Network Ano de publicação: 2005 Tipo de documento: Artigo
...