Prevalence and Genetic Characterization of Toxoplasma gondii in Pet Dogs in Central China
The Korean Journal of Parasitology
; : 125-128, 2015.
Article
em En
| WPRIM
| ID: wpr-130546
Biblioteca responsável:
WPRO
ABSTRACT
The prevalence and genotype of Toxoplasma gondii infection in dogs in Henan Province, Central China was investigated. A total of 125 blood samples were collected from pet dogs during April to June 2013, and all samples were examined by indirect hemagglutination antibody test (IHA) and nested PCR. The overall T. gondii prevalence in pet dogs was 24.0% (30/125), with 20.8% (26/125) in IHA and 10.4% (13/125) in PCR, respectively. No statistical associations were found between animal gender and age and the prevalence of T. gondii infection. Thirteen positive DNA samples were genotyped using 11 PCR-RFLP markers, including SAG1, (3'+5') SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico. Of these, only 2 samples were genotyped with complete data for all loci, and a novel genotype (type III at SAG3 and GRA6 loci, and type I at other loci) was identified. This is the first report of genetic characterization of T. gondii infection in dogs in China.
Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Toxoplasma
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Polimorfismo de Fragmento de Restrição
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Testes de Hemaglutinação
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China
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Toxoplasmose Animal
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Reação em Cadeia da Polimerase
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Prevalência
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Doenças do Cão
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Animais de Estimação
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Genótipo
Tipo de estudo:
Prevalence_studies
Limite:
Animals
País/Região como assunto:
Asia
Idioma:
En
Revista:
The Korean Journal of Parasitology
Ano de publicação:
2015
Tipo de documento:
Article