Differential diagnosis of Trichostrongylus and hookworm eggs via PCR using ITS-1 sequence
The Korean Journal of Parasitology
; : 69-74, 2007.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-131767
Biblioteca responsável:
WPRO
ABSTRACT
Trichostrongylus eggs observed in cellophane-thick smears are difficult, in practice, to distinguish from hookworm eggs. In order to overcome these limitations, a molecular approach was conducted. A Trichostrongylus colubriformis adult worm was obtained from a human in Laos, which was identified morphologically. ITS-1 sequence of this worm was determined, and found to be most similar with that of T. colubriformis among the Trichostrongylus spp. reported so far. Then, this sequence was compared with those of human hookworm species, Ancylostoma duodenale and Necator americanus, and species-specific oligonucleotide primers were designed. Polymerase chain reaction (PCR) using these primers evidenced specifically amplified PCR products of Trichostrongylus sp., A. duodenale and N. americanus from the eggs of each (520 bp, 690 bp, and 870 bp, respectively). A species-specific PCR technique can be developed in order to study the epidemiology of Trichostrongylus spp. and hookworms in endemic areas.
Texto completo:
Disponível
Contexto em Saúde:
Doenças Negligenciadas
Problema de saúde:
Helmintíase
/
Doenças Negligenciadas
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Filogenia
/
Tricostrongilose
/
Trichostrongylus
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Reação em Cadeia da Polimerase
/
DNA de Protozoário
/
Alinhamento de Sequência
/
Necator americanus
/
DNA Intergênico
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
The Korean Journal of Parasitology
Ano de publicação:
2007
Tipo de documento:
Artigo