Effects of Denervation on Erectile Dysfunction: Reduced Erectile Activity Associated with Apoptosis of Smooth Muscle Cells in Rat Corpus Carvernosum / 대한남성과학회지
Korean Journal of Andrology
; : 35-40, 1998.
Artigo
em Coreano
| WPRIM (Pacífico Ocidental)
| ID: wpr-135651
Biblioteca responsável:
WPRO
ABSTRACT
PURPOSE:
Although many clinical observations support the idea that denervation may cause erectile dysfunction, there has been suggested as an underlying cause, resulting in decreased penile tissue and consequently impotence. This study was initiated to investigate whether apoptosis follows denervation of the rat penis. MATERIALS ANDMETHODS:
A total of 40 male Sprague-Dawley rats (10 weeks old) underwent abdominal exploration and unilateral or bilateral cavernous neurotomy. The penises were collected following sacrifice of animals at postoperation day 1, 2, 3, 6, or 10. Sham-operated animals served as controls. Tissues were embedded in paraffin and 3-?3 sections obtained. Deoxyribonucleic acid (DNA) fragmentation was analyzed by an in situ end-labeling method and by gel electrophoresis. Immunohistochemical study was carried out using monoclonal antibodies to p53.RESULTS:
DNA fragmentation was evident in the tissues. In situ labeling revealed that the number of apoptotic bodies increased in tandem with the length of denervation. This finding was confirmed by electrophoretic analysis of DNA. However, there was no difference between the unilaterally and bilaterally treated groups. Immunohistochemical localization showed that p53 expression was increased in tissue sections of denervated animals, whereas little or no expression was shown in normal controls.CONCLUSIONS:
Our results demonstrate that denervation causes apoptotic cell death in the rat penis. It is also suggested that unilateral nerve injury may be sufficient to affect normal erectile function. Further study will elucidate the molecular mechanisms of impotence caused by nerve-damaging operations such as prostatectomy.
Texto completo:
Disponível
Contexto em Saúde:
ODS3 - Meta 3.4 Reduzir as mortes prematuras devido doenças não transmissíveis
Problema de saúde:
Saúde Mental e Transtornos do Comportamento
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Parafina
/
Pênis
/
Prostatectomia
/
DNA
/
Morte Celular
/
Ratos Sprague-Dawley
/
Apoptose
/
Miócitos de Músculo Liso
/
Denervação
/
Eletroforese
Limite:
Animais
/
Humanos
/
Masculino
Idioma:
Coreano
Revista:
Korean Journal of Andrology
Ano de publicação:
1998
Tipo de documento:
Artigo