Correlations in the results of virus neutralization test, hemagglutination inhibition test, and enzyme-linked immunosorbent assay to determine infectious bronchitis virus vaccine potency
Korean Journal of Veterinary Research
; : 189-192, 2016.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-13821
Biblioteca responsável:
WPRO
ABSTRACT
The virus neutralization (VN) test was used to determine potency of the infectious bronchitis (IB) vaccine. The results of VN, hemagglutination inhibition (HI), and enzyme-linked immunosorbent assay (ELISA) were compared with those of the IBV M41. The r² values between VN and HI titers and the ELISA antibody titer were 0.8782 and 0.0336, respectively, indicating a high correlation between VN and HI, but not VN and ELISA. The Cohen's kappa coefficient between the VN titer of 2 log₁₀ and HI titer of 5 log₂ was 0.909. Our results showed that VN could be replaced with HI for testing the potency of IBV M41.
Texto completo:
Disponível
Contexto em Saúde:
Agenda de Saúde Sustentável para as Américas
Problema de saúde:
Objetivo 10: Doenças transmissíveis
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Bronquite
/
Ensaio de Imunoadsorção Enzimática
/
Testes de Inibição da Hemaglutinação
/
Testes de Neutralização
/
Vírus da Bronquite Infecciosa
/
Potência de Vacina
/
Hemaglutinação
Idioma:
Inglês
Revista:
Korean Journal of Veterinary Research
Ano de publicação:
2016
Tipo de documento:
Artigo
