Detection of Glycoproteins (B and D) and Ttymidine Kinase Genes of Herpes simplex virus Type 2 Strain G
Journal of the Korean Society of Virology
; : 99-105, 1999.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-142035
Biblioteca responsável:
WPRO
ABSTRACT
BamHI restriction patters and genomic library of Herpes simplex virus type 2 (HSV-2) stram G were constructed, and locations of the glycoproteins gB and gD, and it genes on the fragments were detected by Southern blot analysis. HISV-2 genomic DNAs were cleaved into twenty-seven fragments by BamHI enzyme in the range of 0.72 to 15.08 (total 150.44 kb), which were cloned into the BamHI site of pBluescript SK(+) to construct genome library of the HSV-2. The library was named by the order of the fragment size from smallest one to largest one. HSV-2 glycoprotein gD gene was located in PHLA2-21 and PHLA2-22 recombinant plasmids, gB gene in PHLA2-24 plasmic, and it gene in PHLA2-11 clone by Southern blot analysis.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fosfotransferases
/
Plasmídeos
/
DNA
/
Glicoproteínas
/
Biblioteca Genômica
/
Southern Blotting
/
Células Clonais
/
Simplexvirus
/
Herpesvirus Humano 2
/
Herpes Simples
Tipo de estudo:
Estudo diagnóstico
Idioma:
Inglês
Revista:
Journal of the Korean Society of Virology
Ano de publicação:
1999
Tipo de documento:
Artigo