Expression and Evaluation of Chikungunya Virus E1 and E2 Envelope Proteins for Serodiagnosis of Chikungunya Virus Infection
Yonsei Medical Journal
; : 828-835, 2008.
Article
em En
| WPRIM
| ID: wpr-153693
Biblioteca responsável:
WPRO
ABSTRACT
PURPOSE: Chikungunya virus (CHIKV) causes endemic or epidemic outbreaks of CHIKV fever, which is a mosquitoe-transmitted viral disease in Africa, India, South-East Asia, and recently Southern Europe. Currently, serological diagnostic tests such as hemagglutination inhibition test (HI test), in-house IgM capture enzyme-linked immunosorbent assays (ELISA), and indirect immunofluorescence test were used for diagnosis of chikungunya fever, which are based on whole virus antigens. MATERIALS AND METHODS: CHIKV E1, and E2 envelope proteins for the CHIKV-specific serodiagnostic reagents for chikungunya fever were expressed in baculovirus expression system. The seroreactivity of recombinant CHIKV E1 and E2 envelope proteins were evaluated using sera panels of patients from Laboratoire Marcel Merieux by indirect IgM capture ELISA. RESULTS: The recombinant CHIKV E1 and E2 envelope protein showed sensitivity of 77.5% and 90%, respectively. The specificities of both CHIKV E1 and E2 envelope proteins were 100%. CONCLUSION: The recombinant CHIKV E1 and E2 envelope proteins could be a useful diagnostic reagent for CHIKV infection.
Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Proteínas Recombinantes
/
Ensaio de Imunoadsorção Enzimática
/
Testes Sorológicos
/
Células Cultivadas
/
Vírus Chikungunya
/
Proteínas do Envelope Viral
/
Baculoviridae
/
Sensibilidade e Especificidade
/
Clonagem Molecular
/
Infecções por Alphavirus
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
En
Revista:
Yonsei Medical Journal
Ano de publicação:
2008
Tipo de documento:
Article