A Study of Surface Physical Properties of New Surfactant Using Synthetic Peptides of Surfactant Protein-B
Journal of the Korean Pediatric Society
; : 1059-1067, 2000.
Article
em Ko
| WPRIM
| ID: wpr-154019
Biblioteca responsável:
WPRO
ABSTRACT
PURPOSE: To produce a new generation of artificial pulmonary surfactant(PS), surfactant protein (SP)-B from human PSwas isolated, and the amino acid sequences of these proteins were studied. Artificial peptides of human SP-B were synthesized. New artificial PS preparations which were cornposed of phospholopids and two artificial synthetic SP-B peptides were made, and the surface physical properties of these new PS preparations were tested. METHODS: The purities of SP-B were assessed by SDS-polyacrylamide gel and the amino acid sequences of these proteins were determined. We synthetized two peptides SP-1 and SP-2 and the amino acid sequences were as follows,' SP-1: RMLPQLVCRLVLRCSMD, SP-2: RMLP- QLVCRLVLRCSM. Surface physical properties of newly artificial PSs, which were composed of a mixture of phospholipid(PL) and SP-1 or SP-2(sample A; PL+SP-1, sample B; PL+SP-2), were measured by surface spreading, adsorption rate, and surface tension-area diagram. RESULTS: The amino acid sequence of human SP-B was obtained. We produced the artificial peptides of SP-B and prepared the new generation PS(sample A and sample B). The order of the superiority of spreading and adsorption rate was SurfactenPalavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Peptídeos
/
Surfactantes Pulmonares
/
Sequência de Aminoácidos
/
Adsorção
/
Absorção
Limite:
Humans
Idioma:
Ko
Revista:
Journal of the Korean Pediatric Society
Ano de publicação:
2000
Tipo de documento:
Article
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Peptídeos
/
Surfactantes Pulmonares
/
Sequência de Aminoácidos
/
Adsorção
/
Absorção
Limite:
Humans
Idioma:
Ko
Revista:
Journal of the Korean Pediatric Society
Ano de publicação:
2000
Tipo de documento:
Article