Using Genotyping to Identify an A1B(weak) Blood Group / 대한수혈학회지
Korean Journal of Blood Transfusion
; : 158-164, 2010.
Article
em Ko
| WPRIM
| ID: wpr-165957
Biblioteca responsável:
WPRO
ABSTRACT
Since an exact ABO blood type match is essential for transfusion therapy, any ABO discrepancies should be resolved prior to the issuing of blood. The authors confirmed the ABO blood group of a 50-year-old male using genotyping. On a routine blood group test, the cell type was A+; however, anti-B was undetected in his serum. To determine the cause of this ABO discrepancy, an adsorption elution test and saliva test were performed. The presence of a weak B substance was suspected despite no evidence of the B antigen on red blood cells. Polymerase-chain-reaction restriction-fragment-length-polymorphism (PCR-RFLP) and sequencing analysis of exons 6 and 7 demonstrated that his blood type was A1Bweak (the A allele tested as the A105 subtype, while the B allele was most similar to the B302 subtype). Again, using genotyping, we subsequently confirmed the A1Bweak blood type in a leukemic patient who was in complete remission.
Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Saliva
/
Leucemia
/
Éxons
/
Adsorção
/
Alelos
/
Eritrócitos
Limite:
Humans
/
Male
Idioma:
Ko
Revista:
Korean Journal of Blood Transfusion
Ano de publicação:
2010
Tipo de documento:
Article