Efficacy of Purified Vi Polysaccharide Typhoid Vaccine
Journal of Veterinary Science
; : 67-70, 2002.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-172834
Biblioteca responsável:
WPRO
ABSTRACT
This experiment was conducted to assess the efficacy of typhoid vaccine newly produced by purifying Vi antigen of Salmonella typhi. With Karber method, LD50 of challenging organism (S. typhi ty2) was determined as 6.31 CFU/mouse, and then the organism was used for the study. With Probits method, ED50 of the vaccine was determined as 0.016 microgram / 0.5 ml / mouse. The ELISA titer (0.5097+/-0.0606) was 4 times in the group treated with high dose (0.25 microgram/0.5ml) as in control (0.1113+/-0.0110). Six major protein bands of 66, 55, 35, 33, 18, and 9 kd were detected in Western blot analysis with serum of a vaccine treated mouse, whereas only one weak band of about 35 kd was detected with serum of a control mouse. We concluded that typhoid vaccine produced by purifying Vi antigen of S. typhi very effectively prevent S. typhi infection in mice.
Texto completo:
Disponível
Contexto em Saúde:
ODS3 - Saúde e Bem-Estar
/
Doenças Negligenciadas
Problema de saúde:
Meta 3.3: Acabar com as doenças tropicais negligenciadas e combater as doenças transmissíveis
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Doenças Negligenciadas
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Zoonoses
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Polissacarídeos Bacterianos
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Salmonella typhi
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Febre Tifoide
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Ensaio de Imunoadsorção Enzimática
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Vacinas Tíficas-Paratíficas
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Modelos Logísticos
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Western Blotting
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Dose Letal Mediana
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Camundongos Endogâmicos BALB C
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Anticorpos Antibacterianos
Tipo de estudo:
Fatores de risco
Limite:
Animais
Idioma:
Inglês
Revista:
Journal of Veterinary Science
Ano de publicação:
2002
Tipo de documento:
Artigo