In Vitro Effect of Fatty Acids Identified in the Plasma of Obese Adolescents on the Function of Pancreatic β-Cells
Diabetes & Metabolism Journal
; : 303-315, 2017.
Article
em En
| WPRIM
| ID: wpr-174848
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND: The increase in circulating free fatty acid (FFA) levels is a major factor that induces malfunction in pancreatic β-cells. We evaluated the effect of FFAs reconstituted according to the profile of circulating fatty acids found in obese adolescents on the viability and function of the murine insulinoma cell line (mouse insulinoma [MIN6]). METHODS: From fatty acids obtained commercially, plasma-FFA profiles of three different youth populations were reconstituted: obese with metabolic syndrome; obese without metabolic syndrome; and normal weight without metabolic syndrome. MIN6 cells were treated for 24 or 48 hours with the three FFA profiles, and glucose-stimulated insulin secretion, cell viability, mitochondrial function and antioxidant activity were evaluated. RESULTS: The high FFA content and high polyunsaturated ω6/ω3 ratio, present in plasma of obese adolescents with metabolic syndrome had a toxic effect on MIN6 cell viability and function, increasing oxidative stress and decreasing glucose-dependent insulin secretion. CONCLUSION: These results could help to guide nutritional management of obese young individuals, encouraging the increase of ω-3-rich food consumption in order to reduce the likelihood of deterioration of β-cells and the possible development of type 2 diabetes mellitus.
Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Plasma
/
Técnicas In Vitro
/
Linhagem Celular
/
Sobrevivência Celular
/
Estresse Oxidativo
/
Diabetes Mellitus Tipo 2
/
Células Secretoras de Insulina
/
Ácidos Graxos
/
Ácidos Graxos não Esterificados
/
Insulina
Tipo de estudo:
Prognostic_studies
Limite:
Adolescent
/
Humans
Idioma:
En
Revista:
Diabetes & Metabolism Journal
Ano de publicação:
2017
Tipo de documento:
Article