Detection of Chemokine Gene Expression Induced by IL-12/IL-2 in Renca Tumor / 대한면역학회지
Korean Journal of Immunology
; : 17-24, 1999.
Article
em Ko
| WPRIM
| ID: wpr-181230
Biblioteca responsável:
WPRO
ABSTRACT
In order to evaluate antitumor rnechanisms of interleukin (IL)-12/IL-2 that has been shown significant tumor suppressive activity on established primary and metastatic Renca tumor, we studied chemokine gene expression induced by direct action of IL- 12/IL-2 or cytokine cascade. IL-12/IL-2 induced gene expression of interferon gamma (IFN-r) and granulocyte monocyte-colony stimulating factor (GM-CSF) in splenocytes, and it induced gene expression of monokine induced by IFN-r (Mig), interferon inducible protein 10 (IP- 10), SDF-1, macrophage inflammatory protein (MIP)-1a, MIP-1B, MIP-2, monocyte chemotactic protein (MCP)-1, and Rantes in tumor mass. However IL-12/IL-2 could not induce these chemokines in tumor mass of GKO mice and Renca cell in vitro. IL- 12 also did not increased chemokine gene expression in Renca cell in vitro, but IFN-r induced gene expression of Mig, IP-10, MCP-1 in Renca cell in vitro. In the chemotaxis assay, culture supernatant of Renca cell stimulated with IFN-r increased splenocyte migration in vitro. All these data suggest IL-12/IL-2 can induce IFN-r-chemokine cascade in tumor mass, and Mig, IP-10, MCP-1 produced from tumor cell may play an important role for initial immune cell migration into tumor mass.
Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Monócitos
/
Expressão Gênica
/
Movimento Celular
/
Quimiotaxia
/
Interleucinas
/
Interferons
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Quimiocina CCL5
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Quimiocinas
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Quimiocina CXCL10
/
Granulócitos
Tipo de estudo:
Diagnostic_studies
Limite:
Animals
Idioma:
Ko
Revista:
Korean Journal of Immunology
Ano de publicação:
1999
Tipo de documento:
Article