Tissue-specific expression of the NOD-like receptor protein 3 in BALB/c mice
Journal of Veterinary Science
; : 173-177, 2014.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-191853
Biblioteca responsável:
WPRO
ABSTRACT
Activation of the innate immune system requires recognition of pathogen-associated molecular patterns, such as NOD-like receptors. The NOD-like receptor protein 3 (NLRP3) inflammasome is involved in induction of the pro-inflammatory cytokine, IL-1beta, and subsequent inflammatory responses. NLRP3 inflammasome plays important roles in the inflammatory and innate immune responses associated with autoimmune/inflammatory syndrome. However, analysis of the tissue distribution and expression profiles in BALB/c mice is still incomplete. In this study, we investigated the tissue distribution and expression pattern of NLRP3 in BALB/c mice to further elucidate its function in innate immunity in this commonly used laboratory animal model. NLRP3 mRNA expression levels and tissue distribution of the protein were investigated by real-time quantitative PCR and immunohistochemical analyses, respectively. NLRP3 mRNA expression was higher in the kidney and inguinal lymph nodes than in other tissues. Cytoplasmic expression of NLRP3 was detected in the epithelial reticular cells of the spleen and thymus, lymphocytes in the inguinal lymph nodes, cardiac muscle cells, cerebral cortex neurons, alveolar macrophages, renal tubule cells and liver sinusoidal endothelial cells. The results of this study will assist investigators in interpreting site-specific functions and roles of NLRP3 in inflammatory responses.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Especificidade de Órgãos
/
RNA Mensageiro
/
Dados de Sequência Molecular
/
Proteínas de Transporte
/
Regulação da Expressão Gênica
/
Análise de Sequência de DNA
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Reação em Cadeia da Polimerase em Tempo Real
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Imunidade Inata
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Camundongos Endogâmicos BALB C
Limite:
Animais
Idioma:
Inglês
Revista:
Journal of Veterinary Science
Ano de publicação:
2014
Tipo de documento:
Artigo