Glucose-Insulin-Potassium Solution Protects Ventricular Myocytes of Neonatal Rat in an In Vitro Coverslip Ischemia/Reperfusion Model
Korean Circulation Journal
; : 234-241, 2015.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-19603
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND AND OBJECTIVES:
The benefit of high glucose-insulin-potassium (GIK) solution in clinical applications is controversial. We established a neonatal rat ventricular myocyte (NRVM) in vitro coverslip ischemia/reperfusion (I/R) model and investigated the effects of GIK solution on suppressing reactive oxygen species (ROS) and upregulating O-GlcNacylation, which protects cells from ischemic injury. MATERIALS ANDMETHODS:
NRVMs were isolated from postnatal day 3-4 Sprague-Dawley rat pups and grown in Dulbecco's modified Eagle's medium containing high glucose (4.5 g/L), fetal bovine serum, and penicillin/streptomycin. The effects of the GIK solution on ROS production, apoptosis, and expression of O-GlcNAc and O-GlcNAc transferase (OGT) were investigated in the coverslip I/R model.RESULTS:
Covering the 24-well culture plates for 3 hr with 12 mm diameter coverslips resulted in the appropriate ischemic shock. Glucose and insulin synergistically reduced ROS production, protected NRVM dose-dependently from apoptosis, and altered O-GlcNAc and OGT expression.CONCLUSION:
The high GIK solution protected NRVM from I/R injury in vitro by reducing ROS and altering O-GlcNacylation.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Choque
/
Transferases
/
Espécies Reativas de Oxigênio
/
Ratos Sprague-Dawley
/
Apoptose
/
Células Musculares
/
Miócitos Cardíacos
/
Glucose
/
Insulina
Limite:
Animais
Idioma:
Inglês
Revista:
Korean Circulation Journal
Ano de publicação:
2015
Tipo de documento:
Artigo