Diarylbutane-type Lignans from Myristica fragrans (Nutmeg) show the Cytotoxicity against Breast Cancer Cells through Activation of AMP-activated Protein Kinase
Natural Product Sciences
; : 21-28, 2017.
Article
em En
| WPRIM
| ID: wpr-198626
Biblioteca responsável:
WPRO
ABSTRACT
In our program to search for new AMP-activated protein kinase (AMPK) activators from plants that exert potential anticancer property, we found that an EtOAc extract of Myristica fragrans (nutmeg) activated AMPK enzyme in human breast cancer MCF-7 cells. Two major diarylbutane-type lignans, macelignan and meso-dihydroguaiaretic acid (MDGA), were isolated as active principles from this extract. Treatment of breast cancer cells with two compounds induced cellular apoptosis, evidenced by cleavage of poly-(ADP-ribose) polymerase (PARP) and Ser 15 phosphorylation of p53. Moreover, macelignan and MDGA significantly inhibited the colony formation of MCF-7 breast cancer cells on soft agar. Intraperitoneal injection of macelignan and MDGA (20 mg/kg) suppressed the tumor growth of 4T1 mammary cancer cells. These results indicate that the chemopreventive effects of two major diarylbutane-type lignans from Myristica fragrans (nutmeg) may be associated with induction of apoptosis presumably through AMPK activation.
Palavras-chave
Texto completo:
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Base de dados:
WPRIM
Assunto principal:
Fosforilação
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Mama
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Neoplasias da Mama
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Apoptose
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Lignanas
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Myristica
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Ágar
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Proteínas Quinases Ativadas por AMP
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Células MCF-7
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Injeções Intraperitoneais
Limite:
Humans
Idioma:
En
Revista:
Natural Product Sciences
Ano de publicação:
2017
Tipo de documento:
Article