Immunohistochemical Study of CD4 , CD8 ICAM-1 and LFA-1 in Experimental Uveitis
Journal of the Korean Ophthalmological Society
; : 687-692, 1998.
Artigo
em Coreano
| WPRIM (Pacífico Ocidental)
| ID: wpr-199374
Biblioteca responsável:
WPRO
ABSTRACT
We have reported an animal model of human anterior uveitis in Lewis rats with bovine melanin associated antigen[BMAA] which has been named as experimental autoimmune anterior uveitis[EAAU]. Immunopathogenesis in this model during different clinical stages was examined by the immunohistochemical detection of CD4 , CD8 , ICAM-1 and LAF-1 which play an important role in immune and inflammatory response. After the separation of eyes, these eyes were sectioned by cryostat at 6micrometer thick sections and stored at -70degreesC. For immunohistochemical study monoclonal antibodies to CD4, CD8, ICAM-1, and LAF-1 were used. An increase of ICAM-1 expression was shown on the epithelial cells of uveal tissues before clinical sign of uveitis and followed by LFA-1 expression. And there was no staining in retinal pigment epithelium and cornea. In conclusion, the immunopathogenesis of EAAU appears to be mediated by T cells and cells expressing ICAM-1, LFA-1.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Uveíte
/
Linfócitos T
/
Uveíte Anterior
/
Antígeno-1 Associado à Função Linfocitária
/
Molécula 1 de Adesão Intercelular
/
Córnea
/
Modelos Animais
/
Células Epiteliais
/
Epitélio Pigmentado da Retina
/
Leucócitos
Limite:
Animais
/
Humanos
Idioma:
Coreano
Revista:
Journal of the Korean Ophthalmological Society
Ano de publicação:
1998
Tipo de documento:
Artigo