Granulocyte-colony stimulating factor decreases the extent of ovarian damage caused by cisplatin in an experimental rat model / 부인종양
Journal of Gynecologic Oncology
; : 328-333, 2014.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-202217
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE:
To investigate whether granulocyte-colony stimulating factor (G-CSF) can decrease the extent of ovarian follicle loss caused by cisplatin treatment.METHODS:
Twenty-one adult female Sprague-Dawley rats were used. Fourteen rats were administered 2 mg/kg/day cisplatin by intraperitoneal injection twice per week for five weeks (total of 20 mg/kg). Half of the rats (n=7) were treated with 1 mL/kg/day physiological saline, and the other half (n=7) were treated with 100 microg/kg/day G-CSF. The remaining rats (n=7, control group) received no therapy. The animals were then euthanized, and both ovaries were obtained from all animals, fixed in 10% formalin, and stored at 4degrees C for paraffin sectioning. Blood samples were collected by cardiac puncture and stored at -30degrees C for hormone assays.RESULTS:
All follicle counts (primordial, primary, secondary, and tertiary) and serum anti-Mullerian hormone levels were significantly increased in the cisplatin+G-CSF group compared to the cisplatin+physiological saline group.CONCLUSION:
G-CSF was beneficial in decreasing the severity of follicle loss in an experimental rat model of cisplatin chemotherapy.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Biomarcadores
/
Fator Estimulador de Colônias de Granulócitos
/
Cisplatino
/
Insuficiência Ovariana Primária
/
Ratos Sprague-Dawley
/
Modelos Animais de Doenças
/
Avaliação Pré-Clínica de Medicamentos
/
Hormônio Antimülleriano
/
Preservação da Fertilidade
/
Folículo Ovariano
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Journal of Gynecologic Oncology
Ano de publicação:
2014
Tipo de documento:
Artigo