Development of monoclonal antibodies against the abnormal prion protein isoform (PrPres) associated with chronic wasting disease (CWD)
Journal of Veterinary Science
; : 429-432, 2012.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-202775
Biblioteca responsável:
WPRO
ABSTRACT
Monoclonal antibodies (mAbs) specific for the abnormal prion protein isoform (PrPres) are indispensable for diagnosing chronic wasting disease (CWD). In this study, eight mAbs were developed by immunizing PrP knockout mice with recombinant elk PrP and an immunogenic PrP peptide. The reactivity of the mAbs to recombinant PrP and the PrP peptide was measured, and their isotypes were subsequently determined. Among them, four mAbs (B85-05, B85-08, B85-12, and B77-75) were shown by Western blotting to recognize proteinase K-treated brain homogenate derived from an elk suffering from CWD.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Estresse Psicológico
/
Encéfalo
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Western Blotting
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Camundongos Knockout
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Doença de Emaciação Crônica
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Anticorpos Monoclonais
Limite:
Animais
Idioma:
Inglês
Revista:
Journal of Veterinary Science
Ano de publicação:
2012
Tipo de documento:
Artigo