Detection of Infectious Bursal Disease Virus by Double Antibody Sandwich ELISA
Journal of Bacteriology and Virology
; : 139-147, 2008.
Artigo
em Coreano
| WPRIM (Pacífico Ocidental)
| ID: wpr-205798
Biblioteca responsável:
WPRO
ABSTRACT
Infectious bursal disease virus (IBDV) is responsible for a highly contagious disease of poultry causing severe immunosuppression in chickens. A double antibody sandwich ELISA (DAS-ELISA) was developed to detect IBDV from clinical samples. Two kinds of anti-IBDV antibodies, monoclonal antibody R63 and chicken anti-IBDV sera, were used for DAS-ELISA. Detection limit of IBDV by DAS-ELISA was approximately 10(2.7) EID(50)/ml. The DAS-ELISA detected IBDV from most (13/14) of vaccine products including mild, intermediate and intermediate-plus types. The DAS-ELISA also detected IBDV from all (19/19) of field Korean isolates including very virulent and intermediate-plus phenotypes. Our results indicate that the DAS-ELISA would provide useful diagnostic tool to detect IBDV from clinical samples as well as rapid quantitative detection of IBDV.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fenótipo
/
Aves Domésticas
/
Vírus
/
Ensaio de Imunoadsorção Enzimática
/
Galinhas
/
Terapia de Imunossupressão
/
Vírus da Doença Infecciosa da Bursa
/
Limite de Detecção
/
Anticorpos Monoclonais
Tipo de estudo:
Estudo diagnóstico
Idioma:
Coreano
Revista:
Journal of Bacteriology and Virology
Ano de publicação:
2008
Tipo de documento:
Artigo