Immunization of BALB/c mice with Brucella abortus 2308DeltawbkA confers protection against wild-type infection
Journal of Veterinary Science
; : 467-473, 2015.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-207358
Biblioteca responsável:
WPRO
ABSTRACT
Brucellosis is a zoonotic disease that causes animal and human diseases. Vaccination is a major measure for prevention of brucellosis, but it is currently not possible to distinguish vaccinated animals from those that have been naturally infected. Therefore, in this study, we constructed the Brucella (B.) abortus 2380 wbkA mutant (2308DeltawbkA) and evaluated its virulence. The survival of 2308DeltawbkA was attenuated in murine macrophage (RAW 264.7) and BALB/c mice, and it induced high protective immunity in mice. The wbkA mutant elicited an anti-Brucella-specific immunoglobulin G response and induced the secretion of gamma interferon. Antibodies to 2308DeltawbkA could be detected in sera from mice, implying the potential for use of this protein as a diagnostic antigen. The WbkA antigen would allow serological differentiation between infected and vaccinated animals. These results suggest that 2308DeltawbkA is a potential attenuated vaccine against 16M. This vaccine will be further evaluated in sheep.
Texto completo:
Disponível
Contexto em Saúde:
Agenda de Saúde Sustentável para as Américas
/
ODS3 - Saúde e Bem-Estar
/
Doenças Negligenciadas
/
ODS3 - Meta 3.3 Acabar com as doenças tropicais negligenciadas e combater as doenças transmissíveis
Problema de saúde:
Objetivo 10: Doenças transmissíveis
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Meta 3.3: Acabar com as doenças tropicais negligenciadas e combater as doenças transmissíveis
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Doenças Negligenciadas
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Zoonoses
/
Brucelose
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteína Estafilocócica A
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Virulência
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Brucella
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Brucella abortus
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Brucelose
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Imunoglobulina G
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Ovinos
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Zoonoses
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Interferons
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Imunização
Limite:
Animais
/
Humanos
Idioma:
Inglês
Revista:
Journal of Veterinary Science
Ano de publicação:
2015
Tipo de documento:
Artigo