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Detection of Helicobacter pylori in Gastric Aspirates Using a Monoclonal Antibody-Based Test
Gut and Liver ; : 30-34, 2013.
Artigo em Inglês | WPRIM (Pacífico Ocidental) | ID: wpr-214014
Biblioteca responsável: WPRO
ABSTRACT
BACKGROUND/

AIMS:

The objective of this study was to evaluate a monoclonal antibody-based test to detect Helicobacter pylori-specific antigen in gastric aspirates from humans.

METHODS:

Sixty-one volunteers were enrolled in the study. All of the subjects underwent a 13C-urea breath test (UBT) before esophagogastroduodenoscopy. Gastric aspirates were analyzed for pH and ammonia and used for polymerase chain reaction (PCR), culture, and monoclonal antibody-based detection of H. pylori. Multiple biopsies of the gastric antrum and body were obtained for a rapid urease test (RUT) and histological evaluation.

RESULTS:

Thirty-six subjects were H. pylori-positive and 25 were H. pylori-negative according to the UBT results. Compared with the H. pylori-negative subjects, H. pylori-positive subjects had a higher pH (4.77+/-1.77 vs 3.49+/-1.30, p<0.05) and ammonia level (1,130.9+/-767.4 vs 184.2+/-126.3, p<0.0001). The sensitivities and specificities of the PCR test, RUT, culture test, and monoclonal antibody-based test were 100% and 72%, 89% and 100%, 47% and 100%, and 78% and 100%, respectively.

CONCLUSIONS:

The monoclonal antibody-based test for diagnosing H. pylori infection in gastric aspirates has increased sensitivity compared with the culture test and specificity as high as that of the RUT. The test may be useful as an additive test for examining gastric aspirates.
Assuntos

Texto completo: Disponível Base de dados: WPRIM (Pacífico Ocidental) Assunto principal: Antro Pilórico / Urease / Biópsia / Testes Respiratórios / Reação em Cadeia da Polimerase / Endoscopia do Sistema Digestório / Helicobacter pylori / Sensibilidade e Especificidade / Helicobacter / Amônia Tipo de estudo: Estudo diagnóstico Idioma: Inglês Revista: Gut and Liver Ano de publicação: 2013 Tipo de documento: Artigo
Texto completo: Disponível Base de dados: WPRIM (Pacífico Ocidental) Assunto principal: Antro Pilórico / Urease / Biópsia / Testes Respiratórios / Reação em Cadeia da Polimerase / Endoscopia do Sistema Digestório / Helicobacter pylori / Sensibilidade e Especificidade / Helicobacter / Amônia Tipo de estudo: Estudo diagnóstico Idioma: Inglês Revista: Gut and Liver Ano de publicação: 2013 Tipo de documento: Artigo
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