Protein Profile Changes in Platelet Concentrates According to Storage and Leukoreduction- Analysis Using Proteomics Technology / 대한진단검사의학회지
The Korean Journal of Laboratory Medicine
; : 53-63, 2008.
Article
em Ko
| WPRIM
| ID: wpr-219028
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND: Knowing how the protein profile of platelet products changes with storage or leukoreduction may give us greater insight into cell physiology and the cause of transfusion reactions other than cytokines and chemokines. METHODS: We filtered four packs of platelet concentrates (PC) within 24 hr of blood collection and after 120 hrs of storage. Four aliquots of each supernatant in PC were obtained: pre-storage+prefiltration, pre-storage+post-filtration, post-storage+pre-filtration and post-storage+post-filtration. Routine chemistry tests and a two-dimensional electrophoresis (2-DE) were performed. The stained images were analyzed and the significant spots were identified using a peptide mass finger printing (PMF) with matrix assisted laser desorption/ionization-time of flight (MALDI-TOF) analysis after trypsin digestion. RESULTS: The protein spots increased with storage and decreased after filtration (P<0.05, prestorage+post-filtration). The spot density of various proteins, including macrophage inflammatory protein-2 alpha, megakaryocyte colony stimulating factor and interleukin-22 changed with storage and leukoreduction. CONCLUSIONS: The database of identified protein spots and their changes produced in this study is a useful basic tool for future studies on the mechanism of transfusion reactions. Further studies should validate the significance of each protein spot.
Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Fatores de Tempo
/
Plaquetas
/
Preservação de Sangue
/
Eletroforese em Gel Bidimensional
/
Transfusão de Plaquetas
/
Espectrometria de Massas por Ionização e Dessorção a Laser Assistida por Matriz
/
Proteoma
/
Proteômica
/
Procedimentos de Redução de Leucócitos
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
Ko
Revista:
The Korean Journal of Laboratory Medicine
Ano de publicação:
2008
Tipo de documento:
Article