Apigenin and Wogonin Regulate Epidermal Growth Factor Receptor Signaling Pathway Involved in MUC5AC Mucin Gene Expression and Production from Cultured Airway Epithelial Cells / 결핵및호흡기질환
Tuberculosis and Respiratory Diseases
; : 120-126, 2014.
Article
em En
| WPRIM
| ID: wpr-224804
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND: We investigated whether wogonin and apigenin significantly affect the epidermal growth factor receptor (EGFR) signaling pathway involved in MUC5AC mucin gene expression, and production from cultured airway epithelial cells; this was based on our previous report that apigenin and wogonin suppressed MUC5AC mucin gene expression and production from human airway epithelial cells. METHODS: Confluent NCI-H292 cells were pretreated with wogonin or apigenin for 15 minutes or 24 hours and then stimulated with epidermal growth factor (EGF) for 24 hours or the indicated periods. RESULTS: We found that incubation of NCI-H292 cells with wogonin or apigenin inhibited the phosphorylation of EGFR. The downstream signals of EGFR such as phosphorylation of MEK1/2 and ERK1/2 were also inhibited by wogonin or apigenin. CONCLUSION: The results suggest that wogonin and apigenin inhibits EGFR signaling pathway, which may explain how they inhibit MUC5AC mucin gene expression and production induced by EGF.
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Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Fosforilação
/
Expressão Gênica
/
Apigenina
/
Fator de Crescimento Epidérmico
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Células Epiteliais
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Receptores ErbB
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Mucinas
Limite:
Humans
Idioma:
En
Revista:
Tuberculosis and Respiratory Diseases
Ano de publicação:
2014
Tipo de documento:
Article