Eotaxin mRNA Expression in Bronchial Mucosa of Patients with Asthma / 결핵
Tuberculosis and Respiratory Diseases
; : 697-704, 1998.
Article
em Ko
| WPRIM
| ID: wpr-229287
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND: Asthma is a chronic inflammatory disease of the airways characterized by a marked infiltration of ecsinophils in the bronchial mucosa. Asthmatic bronchial muosa produces many factors described as king chernotaetic for inflammatory cells. IL-5, RANTES, and MCP-1 alpha are the chemotactic factors for eosinophils, but their roles are controversiaL Recently eotaxin that is a potent eosinophil chernoattracttnt cytokine was detected in a guinea-pig model of allergic airway inflammation, and human eotaxin was cloned. Eotaxin is a specific chemoattractant for eosinophils, but its role in asthma is not confirmed. We examined the in vivo expression of a,taxin in bronchi of asthmatic patients. METHODS: 11 asthmatics and 2 normal controls were enrolled. All subjects were underwent brcnchcscopy with bronchial biopsies in 2nd or 3rd carina. RNA extraction from biopsy samples was done by acid-guanidium method. Semi-quantitaive RT-PCR was done for evaluation of eotaxin mRNA expression. The extent of eosinophil infiltrartion was evaluated by counting the eosinophils in submucosa in HPF of microscope. RESULTS: Eotaxin mRNA expressed in symptomatic, uncontrolled asthma. Steroid inhibited expression of eotaxin mRNA in asthma. Expression of eotaxin mRNA correlated with eosinohil infiltration in bronchial tissues. CONCLUISON: Expression of eotaxin mRNA increases in uncontrolled asthma and eotaxin is involved in the recruitment of eosinophils.
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Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Asma
/
Biópsia
/
Brônquios
/
RNA
/
RNA Mensageiro
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Fatores Quimiotáticos
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Interleucina-5
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Células Clonais
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Quimiocina CCL5
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Eosinófilos
Tipo de estudo:
Prognostic_studies
Limite:
Humans
Idioma:
Ko
Revista:
Tuberculosis and Respiratory Diseases
Ano de publicação:
1998
Tipo de documento:
Article