Immediate-early inducible function in upstream region of junB gene / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 210-213, 2006.
Article
em En
| WPRIM
| ID: wpr-229700
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To analyze the upstream region of radiation-induced junB gene.</p><p><b>METHODS</b>Four plasmids containing 250 bp, 590 bp, 900 bp and 1650 bp, and CAT reporter gene were constructed separately and introduced to L8704 cells. The cells were irradiated with 2 Gy X-rays and incubated at different intervals. Total RNA was extracted from the cells and fluctuation of the CAT mRNA level was assessed by the RNA ratio of CAT/beta-actin measured by quantitative Northern blot hybridization.</p><p><b>RESULTS</b>CAT mRNA expression containing 900 bp and 1560 bp junB promoter remarkably and rapidly increased, and reached its peak 30 min after 2 Gy X-ray irradiation.</p><p><b>CONCLUSIONS</b>590-900 bp fragments located in the upstream region of junB gene play an important role in the early process of cells against radiation.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Plasmídeos
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Efeitos da Radiação
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Fatores de Tempo
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Raios X
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RNA
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RNA Mensageiro
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Cloranfenicol O-Acetiltransferase
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Células Cultivadas
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Regulação da Expressão Gênica
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Northern Blotting
Limite:
Animals
Idioma:
En
Revista:
Biomedical and Environmental Sciences
Ano de publicação:
2006
Tipo de documento:
Article