Study on the genetic stability of immortalized cell line of lymphocyte cell transformed by EB virus after long subculture process / 中华医学遗传学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the genetic stability of an immortalized cell line transformed by Epstein-Barr virus (EBV) after long subculture process.</p><p><b>METHODS</b>In the present study, the genetic stability including chromosome diploidy, karyotypes and microsatellite DNA were evaluated with chromosome banding techniques and microsatellite DNA detection. The telomerase activity of the immortalized cell line was detected by using the telomerase assay kit.</p><p><b>RESULTS</b>From passage 1 to 30, there were no change of the diploidy, karyotypes of chromosome and microsatellite DNA, and the telomerase activity is negative.</p><p><b>CONCLUSION</b>This study indicates that the immortalized cell line remains stable genetically within limited passages.</p>