Effect of 2-methoxyestradiol on cell differentiation of myeloma cell line CZ-1 / 中华血液学杂志
Chinese Journal of Hematology
; (12): 197-199, 2005.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-229872
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the differentiation induction effect of 2-methoxyestradiol (2ME2), an estrogen derivative on myeloma cell line CZ-1.</p><p><b>METHODS</b>The changes of CZ-1 cells in morphology, expression of surface CD49e and quantity of light chain secretion in the supernatant were observed when treated with 0.1 approximately 0.5 micromol/L 2ME2 for 48 h.</p><p><b>RESULTS</b>2ME2 could induce differentiation of CZ-1 cells. The cells appeared decreased in size of nucleus, increased in cytoplasma, decreased in the ratio of nucleus to plasma, decreased in number or disappearance of nucleolus, and thickness and pyknosis of chromatin. The expression of CD49e was increased from (12.20 +/- 1.57)% to (24.80 +/- 1.26)% (P < 0.05). Light chain secretion in the supernatant was increased from (35.97 +/- 2.60) microg/ml to (79.67 +/- 1.88) microg/ml (P < 0.05).</p><p><b>CONCLUSION</b>Low concentrations of 2ME2 could induce differentiation of myeloma cell line CZ-1.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Diferenciação Celular
/
Integrina alfa5
/
Linhagem Celular Tumoral
/
Relação Dose-Resposta a Droga
/
Estradiol
/
Moduladores de Tubulina
/
Citometria de Fluxo
/
Metabolismo
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Hematology
Ano de publicação:
2005
Tipo de documento:
Artigo