Endothelial cells derived from mesenchymal stem cells harbor immunoregulatory effects / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 175-178, 2007.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-230307
Biblioteca responsável:
WPRO
ABSTRACT
This study was purposed to investigate the immunoregulatory effect of endothelial cells derived from mesenchymal stem cells (MSC). The human MSC was induced to differentiate into endothelial cells for one week. The phenotypes were evaluated by flow cytometry, the cell morphologic feature was observed by invert phase-contrast microscope and analysis of capillary formation was performed by using the in vitro angiogenesis kit. The immunoregulatory effect was detected by lymphocyte transformation test. The result indicated that during the differentiation cells grew fast and there was no significant change in the phenotypes, i.e. CD73, CD105, HLA-ABC were positive and CD34, CD80, CD86, HLA-DR, CD31 were negative. Immunofluorescence analysis showed typical expression of the von Willebrand factor. Differentiated MSCs formed capillary-like structure. Endothelial cells derived from MSC also revealed immunosuppressive effect on T cell proliferation in a dose-dependent manner. It is concluded that endothelial cells derived from MSC also harbor immunoregulatory effect on T lymphocytes.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Fator de von Willebrand
/
Linfócitos T
/
5'-Nucleotidase
/
Diferenciação Celular
/
Células Cultivadas
/
Biologia Celular
/
Células Endoteliais
/
Alergia e Imunologia
/
Células-Tronco Mesenquimais
Limite:
Criança
/
Humanos
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2007
Tipo de documento:
Artigo