Prenatal Diagnosis of Duchenne Muscular Dystrophy (DMD) by Multiplex Polymerase Chain Reaction (PCR) with Chorionic Villi / 대한산부인과학회잡지
Korean Journal of Obstetrics and Gynecology
; : 2028-2032, 1999.
Artigo
em Coreano
| WPRIM (Pacífico Ocidental)
| ID: wpr-23040
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE:
To evaluate the efficacy of prenatal multiplex PCR with chorionic villi, in the case of family history of DMD due to exon deletion.METHODS:
DNA was extracted when cells' size are 1x 106 on T- flask surface area reach to 25 cm2 after 2 weeks from sex confirmation. Average DNA concentration was 50-100 ng and multiplex PCR test was performed from DNA extraction.RESULT:
PCR was done for 17 exons devided into 4 groups. Seventeen exons were all amplified with their right size.CONCLUSIONS:
This method is DNA analysis for prenatal diagnosis of DMD with chorionic villi in the family of DMD. This is useful when preimplantation genetic diagnosis is not available.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Diagnóstico Pré-Natal
/
DNA
/
Reação em Cadeia da Polimerase
/
Éxons
/
Córion
/
Vilosidades Coriônicas
/
Diagnóstico Pré-Implantação
/
Distrofia Muscular de Duchenne
/
Reação em Cadeia da Polimerase Multiplex
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Coreano
Revista:
Korean Journal of Obstetrics and Gynecology
Ano de publicação:
1999
Tipo de documento:
Artigo