In vitro study for detecting the effects of Bazhen decoction on proliferation and activation of T lymphocytes / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 855-858, 2010.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-230770
Biblioteca responsável:
WPRO
ABSTRACT
This study was designed to detect the effects of Bazhen decoction on the proliferation and activation of T lymphocytes. T lymphocytes were isolated. The effects of Bazhen decoction on the prolifertion and activation of T lymphocytes, and on the secretion of IFN-gamma IL-2 from T lymphocytes, were detected by MTT, Flow cytometry and ELISA. Results showed that proliferation of T lymphocytes was promoted significantly by different concentration of Bazhen decoction; and after different time, the relationships of "the longer the time, the higher the concentration, and the more enhanced the proliferation" came to be apparent. After 72h, T lymphocytes were activated with different concentration of Bazhen decoction, the rate of CD69+ T cells increased signficantly, the secretion of IFN-gamma and IL-2 increased signficicantly, and the effect exhibited a dose-dependent manner. The results of this epxeriment indicated that Bazhen decoction could promote the proliferation and activation of T lymphocytes.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Medicamentos de Ervas Chinesas
/
Ativação Linfocitária
/
Linfócitos T
/
Células Cultivadas
/
Biologia Celular
/
Proliferação de Células
/
Alergia e Imunologia
/
Citometria de Fluxo
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2010
Tipo de documento:
Artigo