Construction and recovery of chimeric rabies virus expressing envelop proteins E1E2 of hepatitis C / 中华实验和临床病毒学杂志

ABSTRACT

<p><b>OBJECTIVE</b>Construction and recovery of chimeric rabies virus expressing HCV envelop proteins E1E2.</p><p><b>METHODS</b>On the basis of the previously established reverse genetic system CTN-GFP, HCV E1E2 genes were cloned to both replication competent and replication constrained viral vectors based on CTN181 strain and the chimeric viruses CTN-HCV E1E2 and CTNdeltaG-HCV E1E2 were recovered.</p><p><b>RESULTS</b>The result demonstrated that both the chimeric viruses were rescued successfully, had the ability to re-infect normal sensitive cell lines and express HCV E1E2 genes detected in the level of mRNA.</p><p><b>CONCLUSION</b>The establishment of chimeric RVs expressing HCV E1E2 genes provides the evidence that it is feasible to develop novel HCV vaccines based on viral vectors in theory and in practice.</p>