Molecular and epidemiological study on viral diarrhea among infants in Lanzhou / 中华实验和临床病毒学杂志
Chinese Journal of Experimental and Clinical Virology
; (6): 36-38, 2011.
Article
em Zh
| WPRIM
| ID: wpr-231199
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To study the epidemiologic characteristics of viral diarrhea in children under 5 years old in Lanzhou, understand the four major virus in children of distribution.</p><p><b>METHODS</b>In the first hospital of Lanzhou university from Jul 2009 to Jun 2010,we collected 290 stool specimens from children with diarrhea and 114 asymptomatic controls. Rotavirus was detected by ELISA,further strain characterization was carried out by nested PCR. The human calicivirus, astrovirus, adenovirus were detected by RT-multiplex PCR and PCR.</p><p><b>RESULTS</b>At least one of the four viral agents was found in 60% of the specimens. Rotavirus, human calicivirus, adenovirus, and astrovirus were identified in 39.31%, 11.38%, 10.69%, and 4.83% in 290 specimens respectively. Rotavirus G3 was the most prevailing serotype, P [8] was the most common genotype. In the 114 control samples, 7 sample was positived for calicivirus, 5 samples were positived for human adenovirus and 1 sample was positived for astrovirus.</p><p><b>CONCLUSION</b>The results indicated clearly the impact of viral agents causing diarrhea and the importance of long-term systematic surveillance.</p>
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Mamastrovirus
/
Virologia
/
Caliciviridae
/
Adenovírus Humanos
/
China
/
Epidemiologia
/
Rotavirus
/
Diarreia
Tipo de estudo:
Prognostic_studies
Limite:
Child, preschool
/
Female
/
Humans
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Infant
/
Male
País/Região como assunto:
Asia
Idioma:
Zh
Revista:
Chinese Journal of Experimental and Clinical Virology
Ano de publicação:
2011
Tipo de documento:
Article