Study on optimization of expression, purification, properties and biological function of recombinant human sBLyS / 生物工程学报
Chinese Journal of Biotechnology
; (12): 318-322, 2002.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-231326
Biblioteca responsável:
WPRO
ABSTRACT
The prokaryotic expression plasmid pET-30a(+)/sBLyS was constructed and transformed into E. coli BL21 (lambda DE3). The recombinant protein was found to be highly expressed by the plight of soluble part and inclusion body. For the sake of enhancing the proportion of the soluble part, inducement at 16 degrees C for 12 h was ascertained. The expressing product was then purified by Ni2+ affinity chromatography gel. PI of the recombinant human sBLyS(rhsBLyS) is about 7.1-7.3 and it assembles into a homotrimer. The effect of rhsBLyS on B lymphocytes by MTT method told us the B lymphocytes' proliferating capacity dose depended on concentration and also stimulating time of the rhsBLys. With rhsBLyS(2 micrograms/mL) stimulating 3 days, B lymphocytes can proliferate the most.
Texto completo:
Disponível
Contexto em Saúde:
Doenças Negligenciadas
Problema de saúde:
Doenças Negligenciadas
/
Zoonoses
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Temperatura
/
Proteínas Recombinantes
/
Linfócitos B
/
Ativação Linfocitária
/
Fator de Necrose Tumoral alfa
/
Escherichia coli
/
Fator Ativador de Células B
/
Genética
/
Ponto Isoelétrico
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2002
Tipo de documento:
Artigo