Couple production of human calcitonin and rat peptidylglycine alpha-amidation monooxygenase in insect cells / 生物工程学报
Chinese Journal of Biotechnology
; (12): 20-24, 2002.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-231381
Biblioteca responsável:
WPRO
ABSTRACT
Human calcitonin (hCT) is a 32 amino acid peptide hormone that requires C-terminal amidation for full biological activity. Calcitonin has important physiological function in vivo. We describe the couple expression of a synthesized modified human calcitonin(hmCT) gene fused with glutathione-S-transferase and rat peptidylglycine alpha-amidation monooxygenase (PAM) in insect cells infected by recombinant baculovirus GSTCT/PAM. Using Western blotting against hmCT or rat PAM, the GSThmCT fusion protein had been identified as well as the PAM. Following affinity chromatography with glutathione agarose column, the GSThmCT fusion protein produced by insect cells was purified. The purified fusion protein was also interacted with antibody against hmCT. The couple expression of a modification enzyme and its substrate in eucaryotic expression system may be used for producing other biological activity peptides.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas Recombinantes de Fusão
/
Calcitonina
/
Expressão Gênica
/
Células Cultivadas
/
Baculoviridae
/
Biologia Celular
/
Genética
/
Glutationa Transferase
/
Oxigenases de Função Mista
/
Insetos
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2002
Tipo de documento:
Artigo