Cloning of gdnf in the mouse testis and its expression in sertoli cells / 中华男科学杂志
National Journal of Andrology
; (12): 975-978, 2007.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-232029
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To clone the glial cell line-derived neurotrophic factor (GDNF) from the mouse testis, construct the eukaryotic expression vector and transfect this vector into Sertoli cells in order to use the gdnf-transfected Sertoli cells as the feeder layer to cultivate spermatogonial stem cells (SSCs).</p><p><b>METHODS</b>Total RNA was extracted from the testes of normal mature mice and gdnf was cloned and amplified using RT-PCR, inserted into the eukaryotic expression vector and transfected into sertoli cells (TM4 cell line). Immunofluorescence with anti-GDNF antibodies was performed at 40 h following the transfection.</p><p><b>RESULTS</b>gdnf cDNA was cloned successfully, and GDNF expressed after transfected into Sertoli cells.</p><p><b>CONCLUSION</b>This study provides a basis for culturing SSCs with gdnf-transfected Sertoli cells as the feeder layer.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Células de Sertoli
/
Testículo
/
RNA
/
Transfecção
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Expressão Gênica
/
Clonagem Molecular
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Biologia Celular
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Fator Neurotrófico Derivado de Linhagem de Célula Glial
/
Genética
Limite:
Animais
Idioma:
Chinês
Revista:
National Journal of Andrology
Ano de publicação:
2007
Tipo de documento:
Artigo