A Bw12 blood type caused by 278C>T mutation of ABO gene / 中华医学遗传学杂志
Chinese Journal of Medical Genetics
; (6): 482-484, 2012.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-232270
Biblioteca responsável:
WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To determine the serotype and genotype of a sample with ABO blood group discrepancies.</p><p><b>METHODS</b>Serotype was determined with serological method. Sequence specific primer polymerase chain reaction (SSP-PCR) was carried out based on the serotype. Sequences of exons 6 and 7 of ABO gene was analyzed by sequence-based testing (SBT).</p><p><b>RESULTS</b>Completely agglutinated A antigen, half agglutinated B antigen and weak agglutinated anti-B antibody were detected in both erythrocytes and serum, which suggested presence of a ABw serotype. An A/Bw12 genotype was revealed by B subgroup detection. Sequences of exons 6 and 7 were 278CT, 297GA and 467CT, 526CG, 657CT, 703GA, 796CA, 803GC, 930GA, respectively. The genotype fit with A102/B101 except for a nt278 C>T mutation. Blood group antigen gene mutation database (BGMUT) search has confirmed the mutant allele to be Bw12.</p><p><b>CONCLUSION</b>An A102/Bw12 genotype has been found in the Chinese population.</p>
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Antígenos de Grupos Sanguíneos
/
Sistema ABO de Grupos Sanguíneos
/
Tipagem e Reações Cruzadas Sanguíneas
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Genética
/
Genótipo
/
Métodos
/
Mutação
Limite:
Feminino
/
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Medical Genetics
Ano de publicação:
2012
Tipo de documento:
Artigo