DNA prime followed by protein boost enhances the protective efficacy against Schistosoma japonicum infection in mice / 生物工程学报
Chinese Journal of Biotechnology
; (12): 814-822, 2013.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-233198
Biblioteca responsável:
WPRO
ABSTRACT
Schistosomiasis japonica is an endemic, zoonotic disease of major public health importance in China. Vaccination is needed as a complementary approach to the ongoing control programs. In the present study, we determined if the efficacies of DNA vaccine encoding the SjGST and Sj32 asparaginyl endopeptidase protein could be enhanced by boosting with SjGST-32 protein vaccines. Mice were inoculated with a VR1012-SjGST-32 DNA vaccine followed by boosting with rSjGST-32 at 0, 14 and 28 d. Two weeks after the final boost, mice were challenged percutaneously with cercariae. On day 45 following the challenge, all mice were sacrificed and the numbers of recovered worms and hepatic eggs were counted. Moreover, we analyzed the immune response among various vaccination groups. The results showed that DNA vaccine efficacy was enhanced when mice were boosted with protein vaccine. Adult worm and liver egg burdens were reduced 42.3% and 59.6%, respectively. We further found that DNA vaccine followed by boosting with protein significantly increased the IgG titer and T cell proliferation over those seen in mice vaccinated solely with DNA vaccines. Furthermore, the higher level of IFN-gamma expression in the splenetic CD4+ T cell showed that DNA prime-Protein boosting vaccine induced CD4+ Th1-type responses. Thus, DNA vaccine efficacy was significantly enhanced via boosting protein vaccine which might provide a basis for rational application of the Schistosoma vaccine.
Texto completo:
Disponível
Contexto em Saúde:
Agenda de Saúde Sustentável para as Américas
/
ODS3 - Saúde e Bem-Estar
/
Doenças Negligenciadas
Problema de saúde:
Objetivo 10: Doenças transmissíveis
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Meta 3.3: Acabar com as doenças tropicais negligenciadas e combater as doenças transmissíveis
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Doenças Negligenciadas
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Zoonoses
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Schistosoma japonicum
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Proteínas Recombinantes de Fusão
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Esquistossomose Japônica
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Proteínas de Helminto
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Imunização Secundária
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Vacinação
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Vacinas de DNA
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Alergia e Imunologia
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Glutationa Transferase
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Métodos
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2013
Tipo de documento:
Artigo