GFP used as a reporter system for optimization of K562 cell transfection / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1174-1176, 2008.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-234274
Biblioteca responsável:
WPRO
ABSTRACT
This study was aimed to obtain higher efficiency in gene transfection into K562 cells and to study the role of green fluorescence protein (GFP) as a reporter system. Transfection efficiencies with different methods including electroporation and lipofectamine 2000 transfection, were observed and calculated under fluorescent microscopy by using GFP as a reporter system. The results showed that the transfection efficiency with electroporation (10%) was higher than that with lipofectamine 2000 (1%). In conclusion, the electroporation is a more ideal method for introduction of foreign gene into K562 cells. GFP can be used as a reporter system for optimizing transfection of K562 cells.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Transfecção
/
Genes Reporter
/
Eletroporação
/
Células K562
/
Proteínas de Fluorescência Verde
/
Genética
/
Lipossomos
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2008
Tipo de documento:
Artigo